Life-cycle structural safety and reliability analysis methods requires robust calibration and validation procedures based on the results of experimental tests on existing structures. This paper deals with computational modeling and experimental validation of nonlinear finite element analysis of prestressed concrete (PC) structures under shear-dominant failure mechanisms accounting for uncertainties. Structural modeling is based on a bi-dimensional finite element model for plane-stress analysis formulated in accordance with the Modified Compression Field Theory. Validation is performed on the results of a large experimental campaign carried out on PC bridge deck beams extracted from a 50-year-old viaduct after dismantling. Bayesian model updating with structural reliability methods is adopted to inform material and structural properties of the PC bridge deck beams based on laboratory experimental tests. The statistical residual performance is compared with the experimental results of a full-scale load test up to failure